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. 2013 Jan 24;161(3):1278–1290. doi: 10.1104/pp.112.203760

Figure 7.

Figure 7.

Expression of GhFLA genes and immunohistochemical localization of AGPs in fibers of the transgenic cotton plants. A, Quantitative RT-PCR analysis of the expression of six fiber-specific/preferential FLA genes in 10-DPA fibers of GhFLA1 overexpression and RNAi transgenic cotton plants (T2 generation). Three individual T2 plants were sampled for each transgenic line. Error bars show se (n = 3). Independent Student’s t tests demonstrated that there was a significant difference (*P < 0.05) or a very significant difference (**P < 0.01) between transgenic lines and the wild type (WT) . Accession numbers in GenBank are as follows: GhFLA1 (EF672627), GhFLA2 (EF672628), GhFLA4 (EF672630), GhFLA6 (EF672632), GhFLA14 (EF672640), GhFLA15 (EF672641). B, Paraffin sections of 1-DPA cotton ovules used for immunohistochemical assay. Immunolocalization was carried out with antibodies MAC207, JIM8, JIM13, and LM6, using PBS as a negative control. OL1, OL2, and OL3 (OE L3), GhFLA1 overexpression transgenic lines; RL3, RL4, and RL6 (RNAi L6), GhFLA1 RNAi transgenic lines. Bars = 75 µm.