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. 2013 Jan 24;161(3):1278–1290. doi: 10.1104/pp.112.203760

Figure 8.

Figure 8.

Expression of the genes related to cell wall biosynthesis and immunohistochemical localization of pectin and hemicellulose in fibers of the transgenic cotton plants. A, Quantitative RT-PCR analysis of genes related to cell wall biosynthesis in 10-DPA fibers of GhFLA1 overexpression and RNAi transgenic cotton plants (T2 generation). Three individual T2 plants were sampled for each transgenic line. Error bars show se (n = 3). Independent Student’s t tests demonstrated that there was a significant difference (*P < 0.05) or a very significant difference (**P < 0.01) between transgenic lines and the wild type (WT). Accession numbers in GenBank are as follows: GhUER1 (FJ415167), GhUGD1 (FJ415166), GhUGP1 (FJ415164), GhSUS1 (U73588), GhGT47c (ES807557), GhCESE1 (ES812699). B, Paraffin sections of 1-DPA cotton ovules were used for immunohistochemical assay. Immunolocalization was carried out with antibodies CCRC-M1, JIM5, and JIM7, using PBS as a negative control. OL1, OL2, and OL3 (OE L3), GhFLA1 overexpression transgenic lines; RL3, RL4, and RL6 (RNAi L6), GhFLA1 RNAi transgenic lines. Bars = 75 µm.