Figure 8.

SlARF4 is a possible repressor of the SlGLK1 gene. A, SlGLK1 promoter sequence analysis. The promoter region of SlGLK1 was analyzed for putative cis-acting elements. The identified sites are represented by black bars: MYB-binding site (GTTAGTT), AuxRE (TGTCTC), and MYC box (CATGTG). B, Expression pattern of SlGLK1 and SlARF4 monitored by qPCR in down-regulated fruit compared with the wild type at 25 DPA. The relative mRNA level for each gene was normalized with respect to the actin housekeeping gene. The results were expressed using the wild type as a reference for each gene (relative mRNA level 1). WT, Wild type.