Figure 2.

Effect of Fe deficiency on the expression of central oscillator genes. A, Scheme of plant entrainment and treatments. Fifteen-day-old seedlings were treated with Fe-deficient medium (−Fe); after 60 h, seedlings were transferred to continuous light (LL) at ZT 0. Samples were harvested every 3 h for 102 h from ZT 0. B, Quantitative RT-PCR of mRNA expression of CCA1, LHY, PRR9, PRR7, PRR5, GI, TOC1, and ELF4. The white and gray bars indicate subjective light and dark periods, respectively, under continuous light. UBC21 was used as an internal control. Relative expression was calculated by normalization to the mean of total data. Data are expressed as means ± sd from three technical repeats. Two independent experiments showed similar results.