Figure 8.

Effect of Fe deficiency on the circadian clock in central oscillator mutants. Quantitative RT-PCR of mRNA expression of the central oscillator genes GI and TOC1 is shown in central oscillator mutants. The white and gray bars indicate subjective light and dark periods, respectively, under continuous light (LL). Samples were harvested every 3 h from ZT 36 to ZT 78. UBC21 level was used as an internal control. Relative expression was calculated by normalization to the mean of total data. Data are expressed as means ± sd from three technical repeats. Two independent experiments showed similar results.