Figure 5.

Expression profile of plant defense-related genes during powdery mildew infection of Arabidopsis. Three-week-old 35S::PMR4-GFP, pmr4, and wild-type plants were inoculated with the virulent powdery mildew Gc and the nonadapted powdery mildew Bgh. All tests were conducted with rosette leaves. Relative gene expression was determined by quantitative PCR. RNA was isolated from infected leaf tissue and used as template in complementary DNA generation. Gene expression at 0 hpi was used as a reference, and Actin2 expression was used for normalization. Values of 35S::PMR4-GFP represent the means of lines 1 and 2 in biologically independent experiments. *P < 0.05, **P < 0.01 by Tukey’s test. Error bars represent se, and n = 6. A, Relative expression of the salicylic acid-related gene ICS1. B, Relative expression of the salicylic acid-related gene EDS5. C, Relative expression of the jasmonate-related gene COI1. [See online article for color version of this figure.]