Figure 3.

Carbon source allocation into amino acid families. Provision of fully labeled substrates (Gln or Asn) resulted in the redistribution of label to amino acids and was used to establish the source of carbon for each amino acid family. Distribution of label to each amino acid along with carbon composition and corresponding biosynthetic fluxes were used to calculate the fractional contributions of different carbon sources to amino acids (for details, see Supplemental Data Set S4). Amino acids were grouped according to biosynthetic families by averaging. The results of the 21:1 C:N ratio culture are provided in the top panel, with the 13:1 and 37:1 C:N ratios that indicated similar trends provided in the table. The contributions of Gln-derived carbon to the Glu, Asp, and pyruvate amino acid families are highlighted by the shaded regions in the table.