Table III. Fluxes to protein-bound amino acids, acetyl groups, and hexose units.
The fluxes to amino acids from hydrolyzed protein were determined experimentally by a combination of C:N and amino acid analyses, as described in “Materials and Methods,” and resulted in higher flux values for all amino acids in C:N 13:1. The model fluxes were determined relative to the measured fatty acid fluxes determined per two acetyl units (i.e. four acetyl carbons), and carbohydrates representing the remainder of the biomass were determined on a per hexose unit basis. All fluxes are reported as micromoles of metabolite per milligram dry weight.
| Metabolite | 13:1 |
21:1 |
37:1 |
|||
|---|---|---|---|---|---|---|
| Flux | se | Flux | se | Flux | se | |
| Ala | 0.24 | 0.01 | 0.19 | 0.01 | 0.16 | 0.02 |
| Arg | 0.48 | 0.02 | 0.31 | 0.00 | 0.14 | 0.01 |
| Asp + Asn | 0.34 | 0.04 | 0.25 | 0.02 | 0.22 | 0.01 |
| Glu + Gln | 0.31 | 0.02 | 0.32 | 0.01 | 0.25 | 0.01 |
| Gly | 0.43 | 0.01 | 0.33 | 0.01 | 0.23 | 0.01 |
| His | 0.13 | 0.00 | 0.11 | 0.00 | 0.07 | 0.00 |
| Ile | 0.19 | 0.00 | 0.16 | 0.00 | 0.12 | 0.00 |
| Leu | 0.29 | 0.00 | 0.25 | 0.00 | 0.18 | 0.01 |
| Cys | 0.07 | 0.00 | 0.07 | 0.00 | 0.06 | 0.00 |
| Lys | 0.15 | 0.01 | 0.13 | 0.01 | 0.12 | 0.00 |
| Met | 0.06 | 0.00 | 0.08 | 0.01 | 0.06 | 0.01 |
| Phe | 0.27 | 0.01 | 0.22 | 0.01 | 0.13 | 0.00 |
| Pro | 0.22 | 0.00 | 0.18 | 0.00 | 0.13 | 0.00 |
| Ser | 0.11 | 0.02 | 0.10 | 0.01 | 0.06 | 0.00 |
| Thr | 0.12 | 0.01 | 0.11 | 0.01 | 0.08 | 0.00 |
| Tyr | 0.02 | 0.01 | 0.02 | 0.02 | 0.01 | 0.01 |
| Val | 0.24 | 0.00 | 0.21 | 0.00 | 0.15 | 0.00 |
| Carbohydrates | 2.86 | 0.11 | 3.26 | 0.13 | 3.86 | 0.09 |
| Fatty acids | 1.89 | 0.07 | 1.97 | 0.09 | 2.05 | 0.12 |