Table I. Ptr4CL single substrate specificity.
Ptr4CL3, Ptr4CL5, and Ptr4CL17 activity assays were performed with individual hydroxycinnamic acids. Substrate concentrations were fixed at 50 µm. All recombinant 4CLs show the highest reaction rate with 4-coumaric acid. Values represent means ± se of three technical replicates. ND, Nondetectable.
| 4CL | Substrate Specificity |
||||
|---|---|---|---|---|---|
| 4-Coumaric Acid to 4-Coumaroyl-CoA | Caffeic Acid to Caffeoyl-CoA | Ferulic Acid to Feruloyl-CoA | 5-Hydroxyferulic Acid to 5-Hydroxyferuloyl-CoA | Sinapic Acid to Sinapoyl-CoA | |
| nmol min-1 mg-1 protein | |||||
| Ptr4CL3 | 923.14 ± 98.01 | 445.94 ± 36.27 | 729.62 ± 39.4 | 202.14 ± 19.54 | ND |
| Ptr4CL5 | 582.24 ± 82.35 | 301.61 ± 28.97 | 354.65 ± 51.56 | 174.74 ± 37.28 | 4.65 ± 6.58 |
| Ptr4CL17 | ND | ND | ND | ND | ND |