Table II. Ptr4CL multiple substrate specificity.
Ptr4CL3, Ptr4CL5, and Ptr4CL17 activity assays were performed with a mixture of all five hydroxycinnamic acids with each substrate at a final concentration of 50 µm. All recombinant 4CLs show the highest reaction rate with caffeic acid. Values represent means ± se of three technical replicates. ND, Nondetectable.
| 4CL | Substrate Specificity |
||||
|---|---|---|---|---|---|
| 4-Coumaroyl-CoA | Caffeoyl-CoA | Feruloyl-CoA | 5-Hydroxyferuloyl-CoA | Sinapoyl-CoA | |
| nmol min−1 mg−1 protein | |||||
| Ptr4CL3 | 141.30 ± 31.5 | 317.28 ± 11.29 | 29.17 ± 8.74 | ND | ND |
| Ptr4CL5 | 116.28 ± 35.96 | 252.00 ± 16.69 | 0.47 ± 0.67 | 0.47 ± 0.82 | ND |
| Ptr4CL17 | ND | ND | ND | ND | ND |