Table III. Michaelis-Menten kinetics.
The Michaelis-Menten kinetic parameters are shown for Ptr4CL3 and Ptr4CL5 reactions with 4-coumaric, caffeic, ferulic, 5-hydroxyferulic, or sinapic acid. Values represent means ± se of three technical replicates. ND, Nondetectable.
| Acid |
Km |
kcat |
kcat/Km |
|||
|---|---|---|---|---|---|---|
| Ptr4CL3 | Ptr4CL5 | Ptr4CL3 | Ptr4CL5 | Ptr4CL3 | Ptr4CL5 | |
| μm | min−1 | min−1 μm−1 | ||||
| 4-Coumaric acid | 10.51 ± 2.84 | 148.06 ± 14.04 | 44.48 ± 8.86 | 106.83 ± 22.75 | 4.23 | 0.72 |
| Caffeic acid | 10.56 ± 2.44 | 43.51 ± 5.43 | 17.07 ± 3.42 | 43.69 ± 4.64 | 1.43 | 1.004 |
| Ferulic acid | 70.17 ± 4.37 | 133.51 ± 7.02 | 50.33 ± 2.17 | 68.41 ± 17.87 | 0.72 | 0.51 |
| 5-Hydroxyferulic acid | 10.97 ± 1.25 | 193.40 ± 21.54 | 3.08 ± 0.12 | 37.34 ± 8.53 | 0.28 | 0.19 |
| Sinapic acid | ND | 508.20 ± 37.26 | ND | 20.79 ± 5.23 | ND | 0.04 |