Table V. Ptr4CL5 inhibition kinetics.
Inhibition kinetics constants are shown for Ptr4CL5. Values represent means ± se of three technical replicates. ND, Nondetectable.
| Inhibitor | Substrate |
|||||
|---|---|---|---|---|---|---|
| 4-Coumaric Acid |
Caffeic Acid |
Ferulic Acid |
||||
| Kic | Kiu | Kic | Kiu | Kic | Kiu | |
| μm | ||||||
| 4-Coumaric acid | – | ND | 122.70 ± 6.7 | 78.07 ± 10.83 | 27.92 ± 3.7 | ND |
| Caffeic acid | 7.06 ± 3.1 | 49.75 ± 7.8 | – | 55.97 ± 6.05a | 18.47 ± 0.5 | 14.98 ± 0.67 |
| Ferulic acid | 335.54 ± 76.1 | 178.21 ± 60.7 | 518.93 ± 28.80 | 372.06 ± 73.26 | – | ND |
| 5-Hydroxyferulic acid | 48.8 ± 14.5 | 37.48 ± 5.94 | 45.36 ± 1.75 | 306.81 ± 40.59 | 185.78 ± 56.6 | 18.71 ± 4.62 |
| Sinapic acid | 631.72 ± 189.0 | 238.41 ± 44.7 | 2,841.64 ± 101.25 | 157.21 ± 9.35 | 384.85 ± 30.7 | 450.83 ± 4.1 |
a
Here, Kiu = Kis for substrate self-inhibition.