Figure 2.

Changes in protoplast viability and mitochondrial membrane integrity in the presence of 1 m H₂O₂. A, The viability of cassava mesophyll protoplasts after H₂O₂ treatment was estimated by fluorescein diacetate staining. Data are represented as means of six replicates ± sd (more than 300 cells were counted for each experiment per genotype). Values labeled with different letters (a, b, and c) are significantly different by Duncan’s multiple comparison tests at P < 0.05. B, Mitochondrial membrane integrity under 1 m H₂O₂ stress. Cassava protoplasts were stained with rhodamine 123, and the fluorescent signal was observed with a confocal microscope. Bars = 5 μm. [See online article for color version of this figure.]