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. 2013 Mar 15;140(6):1196–1206. doi: 10.1242/dev.087528

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© 2013. Published by The Company of Biologists Ltd

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Fig. 2. — Proliferation and Wnt signaling decreased with age in the postnatal cochlea. (A-C) Cryosections of cochleae from mice administered EdU once daily for 2 days. EdU-labeled TBCs decreased from P3 to P9, and further decreased from P9 to P23. (D) Quantification of EdU-positive TBCs (n=3-10). (E) The number of TBCs (between the lateral cochlear wall and habenula perforata) decreases with age, n=6 for each age. (F) Using RT-PCR and qPCR, cochleae demonstrated a significant decrease in the Wnt target genes (Axin2 and Sp5) (P<0.005 for both) between P2 and P15; experiments were carried out in triplicate. Levels of the hair cell marker (Brn3.1) remained constant (P=0.24). Levels of the supporting cell marker (Sox2) decreased (P<0.05) and those of the outer hair cell gene Prestin increased (P<0.001). (G-J) Axin2^lacZ expression in the postnatal cochlea decreased with age. Scale bars: 25 μm in A-C; 200 μm in G-J. Data are mean±s.d. Asterisk indicates statistical significance.