Fig. 1.

Genetic interaction between Vangl2^Lp and Cfl1^C5 at E9.5. (A) A Vangl2^Lp/+ Cfl^C5/+ embryo, which is indistinguishable from wild type. (B) Cfl1^C5 homozygote. Homozygous mutants are exencephalic and die ∼E11.0. (C) Vangl2^Lp homozygote. Homozygous mutant embryos have an open neural tube caudal to the midbrain-hindbrain junction. (D) Vangl2^Lp Cfl^C5 double mutant. These embryos have a completely open neural tube, a short body axis and abnormal somites. (E-H) Meox1 in situ hybridization (purple) highlights the somites; there are wide somites in the double mutant (H). Insets show a 4× higher magnification of somites. (I) The length to width ratio (LWR) of somites. Somites in Vangl2^Lp mutants are significantly shorter and wider than wild-type or Cfl^C5 embryos (P<0.001). Somites in Vangl2^Lp Cfl^C5 double mutants have significantly reduced LWR compared with wild type or either single mutant (P<0.001); Vangl2^Lp Cfl^C5 double mutants had 20-22 somite pairs and other genotypes had 22-28 at the stage analyzed. Scale bars: 250 μm. Data are mean±s.d.