Fig. 2.

Cfl1^C5 enhances the convergent extension defect in the Vangl2^Lp notochord. (A-D) Projections of flat-mounted E8.0 embryos stained for brachyury (T) (magenta). (A′-D′) Higher magnifications (4×) of midline cells from boxed regions of A-D highlight the arrangement of cells in each genotype. Wild type (A) and Cfl1^C5 (B) have a similar arrangement of T⁺ cells in the midline, whereas the Vangl2^Lp midline (C) is wider and shorter. The Vangl2^Lp Cfl1^C5 mutant midline (D) is wider and shorter than Vangl2^Lp. (E,F) The LWR of the notochordal plate measured by the distribution of cells (E) or in μm (F). Scale bars: 100 μm in A-D; 25 μm in A′-D′. Data are mean±s.d.