Fig. 6.

A 50 amino acid N-terminal domain of PUF-8 is crucial for both protein-protein interactions and functional redundancy with TCER-1. (A) (Left) Yeast two-hybrid assay as in Fig. 4 but using the BD fusions of various PUF-8 N-terminal deletions and the AD fusions indicated above each panel. The regions deleted are illustrated alongside. (Centre) Rescue of the indicated mutant strain by puf-8::gfp transgenes bearing the same deletions used in the yeast two-hybrid assay. The number of worms observed is given in parenthesis. (Right) Expression patterns of the corresponding transgenes in the distal gonad as visualised by GFP fluorescence. The level and pattern of expression were approximately the same for all the transgenes. Transgenic lines bearing deletion of amino acids 174-535 could not be generated. (B) Expression patterns of the PUF-8 C-terminal region (amino acids 144-535) fused to GFP and the nuclear envelope marker mCherry::EMR-1 in worms carrying both transgenes. Scale bars: 20 μm in A; 10 μm in B.