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. 2013 Mar 15;140(6):1353–1363. doi: 10.1242/dev.088351

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© 2013. Published by The Company of Biologists Ltd

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Fig. 4. — Photoconversion of nkx2.5⁺ cells in the ALPM reveals widespread contributions to SHF-derived structures. (A,B) Dorsal images of a 14- to 16-somite stage Tg(nkx2.5:Kaede) embryo before (A) and after (B) photoconversion with ultraviolet (UV) light. (C) Confocal z-stack image of the ventricle (V) and OFT at 72 hpf. (D) Confocal slice of the OFT. Arrowheads and arrows highlight endothelial and smooth muscle precursor cells in the OFT, respectively. Six out of eight photoconverted embryos exhibited red Kaede protein throughout the ventricle and OFT. ss, somite stage; OFT, outflow tract; hpf, hours post-fertilization.