Table 1.
List of Harvested Tumors After Cell Transplantation
| Injection | No. of tumor/animal | Latency (days) | Culture | Passage | H&E | PCR | IHC |
|---|---|---|---|---|---|---|---|
| Gamma-MEFs | 0/1 | N/A | 10% FBS | 2 | — | — | — |
| Canine (parental) fibroblasts | 0/1 | N/A | 10% FBS | 3 | — | — | — |
| mESCs | 2/2 | 43 | mLIF | 9 | Y | — | — |
| ciPSCs-S1 | 1/1 | 28 | FGF2 | 3 | Y | Y | — |
| ciPSCs-S1 | 1/1 | 28 | hLIF | 3 | Y | Y | — |
| ciPSCs-S2 | 1/1 | 34 | FGF2/hLIF | 4 | Y | Y | — |
| ciPSCs-S3 | 1/1 | 34 | FGF2/hLIF | 4 | Y | Y | — |
| ciPSCs-S4 | 2/2 | 22 | FGF2/hLIF | 3 | Y | Y | Y |
| FACS sorted ciPSCs-S2 | 1/2 | 54 | FGF2/hLIF | 13 | Y | Y | Y |
| FACS sorted ciPSCs-S4 | 2/2 | 46 | FGF2/hLIF | 13 | Y | Y | Y |
H&E, hematoxylin and eosin; PCR, polymerase chain reaction; IHC, immunohistochemistry; MEFs, mouse embryonic fibroblasts; mESCs, mouse embryonic stem cells; ciPSCs, canine-induced pluripotent stem cells; FACS, fluorescence-activated cell sorting; FBS, fetal bovine serum; hLIF, human leukemia inhibitory factor; mLIF, murine LIF; FGF2, fibroblast growth factor 2; N/A: not applicable. Y indicates assays for each column are performed.