FIG. 2.

Effect of DSCM pretreatment on hSDSC expansion under hydrogen peroxide (H₂O₂). (A) Cell morphology of hSDSCs expanded on either DSCM or Plastic was shown under phase-contrast microscope after treatment with varying concentrations (0, 0.05, 0.5, and 5 mM) of H₂O₂ for 4 days. Scale bar is 200 μm. (B) The effect of 0.05 mM H₂O₂ on the proliferation of hSDSCs expanded on either DSCM or Plastic was evaluated using proliferation index by flow cytometry. (C) The effect of 0.05 mM H₂O₂ on the proliferation of hSDSCs expanded on either DSCM or Plastic was evaluated using cell count (n=8 flasks each group) by a hemocytometer. (D) The effect of 0.05 mM H₂O₂ on apoptosis of hSDSCs expanded on either DSCM or Plastic was evaluated using flow cytometry. (E) The effect of 0.05 mM H₂O₂ on the cell cycle of hSDSCs expanded on either DSCM or Plastic was evaluated using flow cytometry. Color images available online at www.liebertpub.com/scd