FIG. 5.

Effect of H₂O₂ treatment in an either cell expansion or chondrogenic differentiation phase on expanded SDSC chondrogenic hypertrophy. (A, C) hSDSCs expanded on either DSCM or Plastic with 0.05 mM H₂O₂ treatment were chondrogenically induced in a pellet culture system for 21 days. (B, D) hSDSCs expanded on either DSCM or Plastic were chondrogenically induced in a pellet culture system for 21 days and treated with H₂O₂ for the following 7 days. IHC staining was used to detect collagen X (A, B). Scale bar is 1 mm. Real-time PCR was used to evaluate hypertrophy marker gene expression (COL10A1) (C, D). Data are shown as average±SD for n=4. ***P<0.001 compared with the corresponding DSCM group. ^###P<0.001 compared with the corresponding group without H₂O₂ treatment. ^$$$P<0.001 compared with the corresponding group treated with 0.05 mM H₂O₂. Color images available online at www.liebertpub.com/scd