Figure 3.

Metaphase plates and karyograms of C. albula and C. fontanae showing Chromomycin A₃/DAPI staining, FISH and CGH experiments. Chromomycin A₃ (CMA₃) fluorescent staining (green) and DAPI (blue) staining in C. albula (a) and C. fontanae (b). FISH with the 28S rDNA (300 bp probe) (red), DAPI counterstaining (blue) in C. albula (c). FISH with the 28S rDNA (800 bp probe) (red), DAPI counterstaining (blue) in C. fontanae (d). A set of reciprocal comparative genomic hybridization (CGH) experiments to C. albula chromosomes (e) and C. fontanae chromosomes (f). In both (e, f), the C. albula genomic DNA was labelled in red and the C. fontanae genomic DNA in green. Bar = 5 μm.