Table 3.
Morphologic parameters, food intake and plasma lipid concentrations of mice
| Morphologic parameters |
Mice groups according to dietary lipids |
|||
|---|---|---|---|---|
| LF | HF | HF-ω3PL | HF-ω3TG | |
| Biometric data |
|
|
|
|
| Initial Body weight (g) |
24.1 ± 0.4 |
24.2 ± 0.3 |
23.9 ± 0.3 |
24.5 ± 0.3 |
| Body weight gain (g) |
4.1 ± 0.1 |
4.2 ± 0.5 |
3.1 ± 0.1*$ |
3.7 ± 0.3 |
| Energy Intake (kJ/mouse/d) |
39.1 ± 0.9 |
68.9 ± 0.3$ |
60.2 ± 1.3$ |
59.8 ± 1.3$ |
| Liver weight (g) |
1.39 ± 0.03 |
1.36 ± 0.03 |
1.21 ± 0.04*$ |
1.27 ± 0.04* |
| WAT weight (g) |
0.84 ± 0.05 |
1.03 ± 0.12 |
0.72 ± 0.05* |
0.93 ± 0.11 |
| Gastrocnemius (g) |
0.16 ± 0.0 |
0.16 ± 0.0 |
0.16 ± 0.0 |
0.15 ± 0.0 |
| Plasma lipids |
|
|
|
|
| TAG (mM) |
0.89 ± 0.06 |
0.66 ± 0.09$ |
0.64 ± 0.04$ |
0.57 ± 0.04$ |
| NEFA (mM) |
0.62 ± 0.5 |
0.35 ± 0.02$ |
0.27 ± 0.01$ |
0.37 ± 0.02$ |
| Glucose (mmol/L) |
9.1 ± 0.8 |
8.6 ± 0.5 |
9.4 ± 0.9 |
9.2 ± 0.7 |
| Plasma insulin (pmol/L) |
37.0 ± 6.9 |
36.8 ± 3.0 |
24.5 ± 3.0 |
26.5 ± 4.8 |
| Liver lipids (mg total fatty acids/g tissue) |
30.3 ± 2.9 |
34.0 ± 3.0 |
30.3 ± 0.6 |
33.1 ± 3.7 |
| Lipid peroxidation markers in plasma (nM) |
|
|
|
|
| 4-HHE |
23.3 ± 13.5 |
103.7 ± 36.5 |
88.8 ± 32.0 |
128.0 ± 37.5 |
| 4-HNE | 4.9 ± 0.3* | 13.4 ± 0.5 | 5.9 ± 1.1* | 8.6 ± 0.3* |
(*P < 0.05 vs HF); ($P < 0.05 vs LF), ANOVA followed by Fisher test.
Data are mean ± SEM for n = 8 per group. Abbreviations: WAT, white adipose tissue; TAG, plasma triacyglycerols; NEFA, non-esterified fatty acids; 4-HHE, 4-hydroxy-2-hexenal; 4-HNE, 4-hydroxy-2-nonenal.
For 4-hydroxy-2-alkenals analysis, 3 pools of 300 μL obtained from 3 mice (100 μL per mice) were used for each group (n = 3).