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(A) AAPH (0–40 µmol/egg) were injected into the air chamber of eggs containing 9-day-old (stage HH 35) chick embryos and incubated for 24 hours. (B) Stage HH 35 chick embryos were treated with AAPH (10 µmol/egg) and cultured for 2–48 hours. The experiments were performed in triplicates with 20 eggs assigned for each group. Embryos without heart beat were deemed dead.
