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. 2013 Mar 1;8(3):e57732. doi: 10.1371/journal.pone.0057732

Figure 4. Effect of AAPH on blood islands formation in early chick embryos.

Figure 4

(A) Schematic illustration of primitive streak stage chick embryos treated with AAPH (left side of embryo) and saline (right side of embryo). (B) In situ hybridization of whole-mount chick embryo revealing VE-Cadherin expression and extent of blood island formation. (C) Statistical chart showing the blood islands density of AAPH (5.0 µmol) treated and untreated sides of embryos. Results presented as mean ± S.D (n = 10). Data analyzed using SPSS13.5 software, **p<0.01 compared with control. (D) Magnified appearance of blood islands (white arrow) of left side of embryos treated with AAPH and (E) right side with saline. Scale bars = 1 mm in B and 100 µm in D–E.