Skip to main content
. 2013 Mar 1;8(3):e56222. doi: 10.1371/journal.pone.0056222

Figure 4. Intracellular interaction of the selected VHHs with PCV2 Cap protein.

Figure 4

The white clones in lane A indicate that the bait (pBD-Cap) has no autoactivator activity in yeast. When DNA-BD/Cap was cotransformed into Y2HGold yeast strain with 12 random positive AD/VHH plasmids, all co-transformants appeared blue on both DDO/X/A and QDO/X/A plates (Figure 4, lanes B-M). In the control group (Figure 4, lane N), the pGBKT7-53 and pGADT7-T co-transformants (positive control) appeared blue on both DDO/X/A and QDO/X/A plates, whereas pGBKT7-Lam and pGADT7-T co-transformants (negative control) appeared white on the DDO/X/A plate and did not grow on the QDO/X/A plate.