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| Primer | Primer Sequence (5′-3′) |
| CALL01 | GTCCTGGCTGCTCTTCTACAAGG |
| CALL02 | GGTACGTGCTGTTGAACTGTTCC |
| VHH-up | TTCCACCCAAGCAGTGGTATCAACGCAGAGTGGGAGTCTGGRGGAGG |
| VHH-down | GTATCGATGCCCACCCTCTAGAGGCCGAGGCGGCCGACATGGAGACGGTGACCWGGGT |
| T7 | TAATACGACTCACTATAGGG |
| 3′AD | AGATGGTGCACGATGCACAG |
| VHH-Cold-F | GGATCCATGCAGGTGCAGCTGGTGGAGTCTGGRGGAGG |
| VHH-Cold-R | GTCGACTTATGTGGAGACGGTGACCWGGGT |
| Cap-F | CGCGGATCCATGACGTATCCAAG |
| Cap-R | AAAAGTCGACCTTAATGGTTAAGT |
