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Effect of OtaL on the affinity of OtaS for 3-PGA. The OtaS homotetramer is similarly activated by 3-PGA and FBP, whereas the mutant OtaS/OtaLD171A heterotetramer has a higher affinity for 3-PGA than FBP. The plots show the activation of OtaS (A) and OtaS/OtaLD171A (B) by 3-PGA (▪) and FBP (·).
