Fig. 2.

Common EcR and ecdysone sensitive target genes with identical expression responses, same polarity expression targets. a Hierarchical clustering revealed a subset of differentially expressed genes which share the same expression response, when either the hormone or the receptor are removed. Clear resolution of down-regulated and up-regulated nodes where observed. Panels B and C. show line graphs of gene expression changes of the common, same polarity target genes between WT to mutant categories. The down-regulated node (B) contains 204 genes that represent targets that are normally activated. The up-regulated node (C) contains 293 genes that represent targets that are normally repressed. This explicitly shows that the hormone and receptor simultaneously function as both an activation signal as well as a repression signal upon distinct subsets of target genes