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Effects of RDD, PHD, and CSD on expression of OPG and RANKL mRNA. A) In situ hybridization of OPG in OB and MSC; B) In situ hybridization of RANKL in OB and bMSC; C) Ratio of RANKL/OPG mRNA expression, RANKL and OPG mRNA expression data were obtained from panels A and C of this figure, see details in the Methods section of the text, (a, p < 0.01, b, p < 0.05). All the experiments were repeated three times.
