Table 2. Extension primer sequences.
| Gene | Mutation | Extension primers (5′→3′) | Concentration* (pmol/μl) | Primer size (bp) | WT allele (peak color) | MT allele (peak color) | |
| GJB2 | c.235delC | F | ACGATCACTACTTCCCCATCTCCCACATCCGGCTATGGGCC | 4 | 41 | C (Black) | T (Red) |
| MT-RNR1 | m.1555A>G | F | TTAACTAAAACCCCTACGCATTTATATAGAGGAG | 0.07 | 34 | A (Green) | G (Blue) |
| SLC26A4 | c.439A>G | F | TTAATAACTGATTAATTGTTAGAGACTTTTTTTCCCCAGGA CCTTTTCCAGTGGTGAGTTTA | 0.05 | 62 | A (Green) | G (Blue) |
| SLC26A4 | c.919-2A>G | F | AAGTTCAGCATTATTTGGTTGACAAACAAGGAATTATTAAA ACCAATGGAGTTTTTAACATCTTTTGTTTTATTTC | 0.03 | 76 | A (Green) | G (Blue) |
| SLC26A4 | c.1149+3A>G | R | TATGTTTTTTTCCTGTTTCCAGCCCTATAAAACCAGTTCAGCA AAAGGGCACCCA | 0.07 | 55 | A (Red) | G (Black) |
| SLC26A4 | c.1229C>T | F | CCTTTGGGATCAGCAACATCTTCTCAGGATTCTTCTCTTGTTTT GTGGCCACCACTGCTCTTTCCCGCA | 6.67 | 69 | C (Black) | T (Red) |
| SLC26A4 | c.2168A>G | F | GGTTCTTTGACGACAACATTAGAAAGGACACATTCTTTTTGACG GTCC | 0.07 | 48 | A (Green) | G (Blue) |
*
Final concentration in the reaction mixture.