Table 3.
Candidate genes with annotations from the SwissProt (SP) database that are involved in environmental stress tolerance, hypoxia, and sulfide metabolism
| Category | Gene description | Contig ID | Accession number | Percent coverage | E-value | Number of contigs |
|---|---|---|---|---|---|---|
| General stress responses | ||||||
| Stress-activated protein kinase signaling pathways [69] |
Stress-activated protein kinase (JNK3) |
comp49081_c0_seq1 |
P53779 |
97 |
2.71E-63 |
1 |
| Stress-activated protein kinase kinase (SAPKK4) |
comp30986_c0_seq1 |
O14733 |
96 |
2.43E-153 |
1 |
|
| Highly inducible heat shock proteins [70,71] |
Hsp 70 |
comp24087_c0_seq1 |
P27541 |
92 |
3.65E-103 |
1 |
| comp34171_c0_seq1 |
Q91233 |
98 |
1.48E-25 |
2 |
||
| Hsp A2 |
comp24673_c0_seq1 |
P54652 |
88 |
1.47E-54 |
1 |
|
| Hsp A4 |
comp12212_c0_seq1 |
Q61316 |
91 |
3.38E-158 |
1 |
|
| comp7589_c0_seq1 |
P34932 |
79 |
<1.00E-180 |
2 |
||
| Hsp 90 |
comp48547_c0_seq1 |
P07900 |
77 |
7.45E-61 |
2 |
|
| comp5586_c0_seq1 |
Q4R4P1 |
76 |
<1.00E-180 |
1 |
||
| comp32336_c0_seq1 |
O61998 |
77 |
8.80E-33 |
3 |
||
| Constitutive heat shock proteins [70,71] |
Hsp83 |
comp28290_c0_seq1 |
P12861 |
95 |
1.42E-43 |
3 |
| Hsc20 |
comp17434_c0_seq1 |
Q8K3A0 |
64 |
3.26E-60 |
1 |
|
| Hsc70 |
comp29081_c0_seq1 |
Q9U639 |
80 |
3.69E-88 |
1 |
|
| Hsc71 |
comp7828_c0_seq1 |
P08108 |
98 |
<1.00E-180 |
4 |
|
| Small heat shock proteins [70,71] |
Hsp β8 |
comp23233_c0_seq1 |
Q9UJY1 |
67 |
6.80E-66 |
1 |
| Oxidative stress responses | ||||||
| Antioxidant systems [72,73] |
Catalase |
comp3837_c0_seq1 |
Q9PT92 |
93 |
<1.00E-180 |
1 |
| (Cu & Zn) superoxide dismutase |
comp21322_c0_seq1 |
Q751L8 |
61 |
2.29E-30 |
1 |
|
| comp496_c0_seq1 |
O73872 |
87 |
3.87E-88 |
2 |
||
| comp17555_c0_seq1 |
P82205 |
64 |
2.05E-33 |
1 |
||
| comp28438_c0_seq1 |
Q9C0N4 |
46 |
2.83E-09 |
1 |
||
| (Mn) superoxide dismutase |
comp29573_c0_seq1 |
P41978 |
57 |
6.78E-51 |
1 |
|
| comp1897_c0_seq1 |
P07895 |
86 |
5.13E-131 |
1 |
||
| Glutathione peroxidases |
comp37533_c0_seq1 |
Q4AEH3 |
61 |
6.22E-27 |
2 |
|
| comp18221_c0_seq1 |
Q4RSM6 |
90 |
5.39E-121 |
1 |
||
| comp559_c0_seq1 |
Q4AEI2 |
80 |
9.44E-87 |
1 |
||
| comp841_c0_seq1 |
P00435 |
81 |
8.33E-86 |
1 |
||
| Thioredoxin and glutathione reductase |
comp2236_c0_seq1 |
Q86VQ6 |
85 |
<1.00E-180 |
1 |
|
| Thioredoxin |
comp26993_c0_seq1 |
Q9BDJ3 |
72 |
4.09E-16 |
1 |
|
| comp572_c0_seq1 |
Q9DGI3 |
90 |
7.86E-20 |
1 |
||
| Methallothioneins [73] |
Metal-responsive element-binding transcription factor 2 |
comp17857_c0_seq1 |
Q02395 |
70 |
3.28E-164 |
1 |
| Hypoxia induced responses | ||||||
| Transcription factors [74] |
Hypoxia-inducible factor 1α |
comp436_c1_seq1 |
Q9YIB9 |
76 |
1.65E-110 |
1 |
| comp2126_c0_seq1 |
Q98SW2 |
78 |
<1.00E-180 |
3 |
||
| Oxygen transport [74] |
Erythropoietin |
comp50800_c0_seq1 |
Q5IGQ0 |
90 |
7.05E-47 |
1 |
| Hemoglobin β chain |
comp12875_c0_seq1 |
P84652 |
87 |
1.01E-78 |
1 |
|
| Myoglobin |
comp390_c1_seq1 |
Q9DGJ1 |
91 |
5.58E-72 |
1 |
|
| Aerobic/anaerobic metabolism [74] |
Malate dehydrogenase |
comp11493_c0_seq1 |
P11708 |
83 |
3.41E-37 |
1 |
| Succinate dehydrogenase |
comp898_c0_seq1 |
Q7ZVF3 |
94 |
<1.00E-180 |
2 |
|
| Citrate synthase |
comp44107_c0_seq1 |
Q91V92 |
77 |
6.21E-39 |
1 |
|
| Phosphoglycerate mutase |
comp703_c0_seq1 |
P18669 |
94 |
6.82E-163 |
1 |
|
| Phosphoglycerate kinase |
comp21645_c0_seq1 |
Q60HD8 |
76 |
2.87E-163 |
1 |
|
| α-enolase |
comp324_c0_seq1 |
Q9PVK2 |
96 |
<1.00E-180 |
1 |
|
| Lactate dehydrogenase (A chain, B chain, C chain) |
comp4088_c0_seq1 |
Q92055 |
99 |
<1.00E-180 |
1 |
|
| comp192_c0_seq1 |
P20373 |
98 |
<1.00E-180 |
1 |
||
| comp17481_c0_seq1 |
Q06176 |
97 |
7.12E-128 |
2 |
||
| Glycogen phosphorylase |
comp28770_c0_seq1 |
Q9XTL9 |
79 |
2.43E-178 |
3 |
|
| Metabolic rate suppression [74] |
α-tropomyosin |
comp1635_c0_seq2 |
P84335 |
99 |
4.56E-56 |
2 |
| comp1488_c0_seq1 |
P13105 |
96 |
1.61E-37 |
1 |
||
| Myosin heavy chain |
comp37734_c0_seq1 |
Q63357 |
87 |
2.22E-41 |
1 |
|
| Insulin-like growth factor binding protein 1 |
comp12811_c0_seq1 |
P24591 |
55 |
5.49E-43 |
1 |
|
| Sulfide detoxification | ||||||
| Sulfide metabolism and toxicity [75-77] |
Sulfide:quinone oxidoreductase |
comp1919_c0_seq1 |
Q9Y6N5 |
85 |
<1.00E-180 |
1 |
| Sulfite oxidase |
comp25579_c0_seq1 |
P07850 |
78 |
2.59E-93 |
2 |
|
| Sulfur dioxygenase (ETHE1) |
comp1681_c0_seq1 |
Q9DCM0 |
78 |
3.54E-104 |
2 |
|
| Thiosulfate sulfurtransferase (Rhodanese) |
comp2624_c0_seq1 |
Q8NFU3 |
62 |
2.19E-28 |
2 |
|
| comp12736_c0_seq1 |
Q3U269 |
71 |
1.22E-176 |
1 |
||
| Mercaptopyruvate sulfurtransferase |
comp1051_c1_seq7 |
P97532 |
74 |
1.81E-15 |
1 |
|
| Cytochrome c oxidase complex (complex III subunit 6, subunit 3) |
comp513_c0_seq3 |
P07919 |
84 |
1.75E-18 |
1 |
|
| comp4_c0_seq1 | Q96133 | 93 | 3.16E-141 | 1 | ||
For each candidate genes, we reported the database and accession number, percent similarity and E-value, as well as the total number of contigs that matched to a specific database record.