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. 2013 Mar 1;8(3):e57932. doi: 10.1371/journal.pone.0057932

Figure 9. STHdhQ111/Q111 cells exhibit an increase in autophagy activity.

Figure 9

A–B, The increase of LC3-I into LC3-II is a typical indicator of the autophagy. The total amount of LC3 including type I and II is significantly higher in STHdhQ7/Q7 cells than STHdhQ111/Q111 cells. In addition, the ratio of type II to type I seems to be greater in STHdhQ111/Q111 cells than in STHdhQ7/Q7 cells. n = 4. Data shown are mean ± SE. *P<0.05 vs. STHdhQ7/Q7. C, Cells were transfected with GFP-LC3 (microtubule-associated protein 1 light chain 3) and 24 h later GFP fluorescence images were monitored. LC3-I is a cytosolic form of LC3 and LC3-II is a membrane-bound form of LC3 that is implicated in autophagy. The localization of GFP-LC3 is the diffused cytosolic pattern in most of STHdhQ7/Q7 cells, while STHdhQ111/Q111 cells exhibit punctuate pattern of GFP-LC3 that indicates the active autophagic vesicles.