Table 1. Summary of the different combinations tested and number of replicates (N) for each.
IIa or IIb | |||||||
Exp. Choice 1 | Exp. Choice 2 | ||||||
Target animalTested animal | Male | Female C/D0 | Male/Female C/D0 | Male/Female D2–4 | FemaleC/D0/Female D2–4 | FemaleC/D0/Female D1 | |
I | Male | N = 20 | N = 20 | N = 20 | N = 20 | N = 25 | N = 10 |
Female C/D0 | N = 20 | N = 20 | N = 20 | ||||
Female D2–4 | N = 20 |
(C/D0: di-ecdysis and beginning of pre-ecdysis (no calcium plates), D1: middle of pre-ecdysis (appearance of calcium plates), D2–4: end of pre-ecdysis (advanced calcium plates), see fig. 1).