Table 3. PyroCleaning results for mtDNA COI amplicons generated from community samples of Collembola from two forest sites on the island of Tenerife.

	Site 1			Site 2
	MID7	MID8	MID9	MID10	MID11	MID12
Total read count
Raw reads	11,249	11,479	9,234	7,583	11,426	11,584
Reads above min. length	7,311	7,613	6,008	5,255	7515	8,165
Step 3	7,311 [100]	7,613 [100]	6,007 [100]	5,255 [100]	7,515 [100]	8,165 [100]
Step 4	4,492 [61]	4,697 [62]	3,501 [58]	3,276 [62]	4,289 [57]	4,923 [60]
Step 5	3,644 [50]	3,815 [50]	2,879 [48]	2,844 [54]	3,540 [47]	4,114 [50]
Step 6	3,596 [49]	3,764 [49]	2,828 [47]	2,744 [52]	3,409 [45]	4,006 [49]
Unique read count
Reads above min. length	4,742	4,962	4,067	3,356	5,234	5,473
Step 3	450 [9.5]	427 [8.6]	381 [9.3]	304 [9.1]	493 [9.4]	504 9.2]
Step 4	252 [5.3]	231 [4.7]	215 [5.3]	170 [5.1]	289 [5.5]	287 [5.2]
Step 5	27 [0.57]	27 [0.54]	30 [0.74]	44 [1.31]	49 [0.94]	46 [0.84]
Step 6	17 [0.36]	17 [0.34]	19 [0.46]	20 [0.60]	20 [0.38]	21 [0.38]

Each site has been amplified in triplicate. 62,825 raw reads were generated on 1/2 of a 454 plate from a total of 103,850 raw reads (the remainder of raw reads belonged to another experiment). Raw reads had a maximum length of 521 bp and an average length of 343 bp. Steps 3–6 are summarised in the text. Numbers in brackets represents sequence reduction as the % of raw reads above a minimum length of 170 nucleotides.