Skip to main content
. 2013 Mar 1;8(3):e57634. doi: 10.1371/journal.pone.0057634

Figure 1. S. marcescens PigP positively regulates prodigiosin production.

Figure 1

A. Complementation analysis of a pigP mutant strain. The vector is pMQ132, and ppigP refers to pMQ221 (Table 1). Error bars = one standard deviation. * = p<0.05 compared to WT by ANOVA with Tukey’s post-test. B. Genetic organization of the chromosome proximal to the pigP gene including the predicted pigP promoter. Enumerated bars under the genes indicate the regions amplified for operon analysis in panel D. C. Growth curve analysis shows similar growth rates for the WT and isogenic pigP mutant strains. The average of four biological replicates is shown. D. Analysis of the pigP operon supports that pigP is in a polycistronic message with SMA3565 and SMA3566. RNA isolated from stationary phase cells was treated with reverse transcriptase (+RT) or without reverse transcriptase (−RT) as a negative control. Positive control DNA and experimental samples were assessed with PCR for the presence of amplicons internal to pigP as a positive control and that span the genes indicated in the figure. Regions amplified by primers are indicated in by numbered brackets in panel B. Primers for analysis of pigP-SMA3565 co-expression extend 144 base pairs into the SMA3565 open frame; those for SMA3565-SMA3566 extend 407 base pairs into the SMA3566 open reading frame.