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. 2013 Mar 1;8(3):e57634. doi: 10.1371/journal.pone.0057634

Figure 6. PigP is necessary for hemolysis in laboratory and clinical isolates. A.

Figure 6

. Hemolytic strains grown on TSA plates with sheep blood show a zone of clearing around colonies indicative of hemolysis. Isogenic pigP mutant strains show highly reduced zones of hemolysis. B. The hemolysis defect of pigP mutants can be complemented by wild-type pigP on a plasmid (pMQ221); vector refers to pMQ132. C. Arabinose inducible expression of swrW is sufficient to restore hemolysis to the pigP mutant. The swrW gene was expressed from plasmid pMQ367 (pswrW), and vector refers to pMQ125. D. Mutation of pigP reduces the hyper-hemolytic phenotypes of crp and hexS mutants.