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. 2013 Mar 1;8(3):e57634. doi: 10.1371/journal.pone.0057634

Table 2. Plasmids used in this study.

Plasmid Description Reference or source
pMal-C2 Maltose binding protein fusion construct New England Biolabs
pStvZ3 oriR6K lacZ nptII promoter probe [35]
pMQ118 suicide vector nptII, rpsL, oriT, URA3, CEN6/ARSH4 [73]
pMQ124 oriColE1, oripRO1600, aacC1, PBAD-lacZa, oriT, URA3, CEN6/ARSH4 [73]
pMQ125 orip15a, oripRO1600, aacC1, PBAD-lacZa, oriT, URA3, CEN6/ARSH4 [73]
pMQ131 oripBBR1, aphA-3, Plac-lacZa, oriT, URA3, CEN6/ARSH4 [73]
pMQ132 oripBBR1, aacC1, Plac-lacZa, oriT, URA3, CEN6/ARSH4 [73]
pMQ179 pMQ118 with internal pigP fragment This study
pMQ196 pMQ118 with internal hexS fragment This study
pMQ200 oriR6K, nptII, PBAD-lacZa, oriT, URA3, CEN6/ARSH4 [73]
pMQ212 pMQ125 with the pigP open reading frame This study
pMQ242 pMQ124+ His8-CRP [35]
pMQ248 pMQ131+ PflhD-lacZ (flhD promoter) This study
pMQ253 pStvZ3+ PpigP (pigP promoter) This study
pMQ268 pStvZ3+ pigA internal fragment [35]
pMQ272 pStvZ3+ hexS internal fragment [22]
pMQ302 pMQ124+ His9-pigP This study
pMQ367 pMQ125+ swrW [22]
pMQ368 pMQ200+ swrW This study
pMQ376 oripBBR1-based plasmid with PswrW-tdtomato reporter [22]
pMQ402 pMAL-C2+ hexS (MBP-HexS fusion construct) [63]