Table 1.
Histological and immunohistochemical analysis
| Author | Year | Journal | Fixation | Decalcification | Histological analysis | ||||
|---|---|---|---|---|---|---|---|---|---|
| H/E | Safranin-O | Immunohistochemistry | Toluidine blue | Alcian blue | |||||
| Campbell and Ranawat [3] | 1966 | J Trauma | – | – | – | – | – | – | – |
| Chiroff and Cooke [4] | 1975 | J Trauma | Formaldehyde 10% | 5% nitric acid and 70% alcohol | Yes | – | – | – | – |
| Green and Banks [8] | 1953 | JBJS | – | – | – | – | – | – | – |
| King [9] | 1932 | JBJS | – | – | – | – | – | – | – |
| Koch et al. [10] | 1997 | KSSTA | Formalin 4% | – | – | – | Yes | – | Yes |
| Linden and Telhag [14] | 1977 | Acta Orthop Scand | Formalin 10% (buffered) | 40% formic acid and 20% sodium citrate | Yes | Yes | – | Yes | – |
| Milgram [16] | 1978 | Radiol | – | – | Yes | – | – | – | – |
| Portigliatti Barbos et al. [20] | 1985 | Ital J Orthop Traumatol | Alcohol | None | – | – | – | – | – |
| Uozumi et al. [28] | 2009 | AJSM | Formalin 10% | EDTA | Yes | – | – | – | – |
| Yonetani et al. [31] | 2010 | KSSTA | Formalin 10% | – | Yes | Yes | – | – | – |
| Yonetani et al. [30] | 2010 | Arthroscopy | – | – | Yes | – | – | – | – |
| Author | Year | Journal | Histological analysis | Immunochemical analysis | Additional comments | ||||
|---|---|---|---|---|---|---|---|---|---|
| Von Kossa stain | Gomorri trichrome stain | Goldner stain | Tetracycline Labeling | Collagen Type I | Collagen Type II | ||||
| Campbell and Ranawat [3] | 1966 | J Trauma | – | – | – | – | – | – | Staining techniques not reported |
| Chiroff and Cooke [4] | 1975 | J Trauma | – | – | – | – | – | – | – |
| Green and Banks [8] | 1953 | JBJS | – | – | – | – | – | – | Staining techniques not reported |
| King [9] | 1932 | JBJS | – | – | – | – | – | – | Staining techniques not reported |
| Koch et al. [10] | 1997 | KSSTA | Yes | Yes | – | – | Yes | Yes | – |
| Linden and Telhag [14] | 1977 | Acta Orthop Scand | – | – | Yes | Yes | – | – | – |
| Milgram [16] | 1978 | Radiol | – | – | – | – | – | – | – |
| Portigliatti Barbos et al. [20] | 1985 | Ital J Orthop Traumatol | – | – | – | Yes | – | – | – |
| Uozumi et al. [28] | 2009 | AJSM | – | – | – | – | – | – | – |
| Yonetani et al. [31] | 2010 | KSSTA | – | – | – | – | – | – | – |
| Yonetani et al. [30] | 2010 | Arthroscopy | – | – | – | – | – | – | – |
H/E = hematoxylin and eosin; JBJS = Journal of Bone and Joint Surgery; KSSTA = Knee Surgery, Sports Traumatology, Arthroscopy; AJSM = American Journal of Sports Medicine.