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. 2013 Feb 5;28(2):241–259. doi: 10.1007/s10539-013-9364-4

Fig. 1.

Fig. 1

Relationship between sequencing read-length and our ability to classify sequences using the RDP Classifier, a popular taxonomic assignment method based on oligonucleotide frequencies (Wang et al. 2007). Simulated sequences were generated from 16S genes to represent the complete sequence between the 515F/806R primers (the “full amplicon”) or shorter 150 or 100 base pair reads from the 515f forward primer