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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Biomaterials. 2012 Feb 21;33(14):3756–3769. doi: 10.1016/j.biomaterials.2012.01.054

Fig. 11.

Fig. 11

Cellular immune responses measured post-challenge. (a) IFN-γ ELISPOT. IFN-γ secreting cell spots after stimulation with inactivated PR8 virus, PR8 hemagglutinin-specific MHC-I and MHC-II peptides. *: not detected. (b–c) In vitro antibody secreting cell responses (ASC). Antibody levels secreted into culture supernatants of bone marrow (b) and spleen cells (c). Bone marrow and spleen samples were collected from individual mice (n = 4 each group) at day 4 post-challenge. Single cell suspensions were prepared and cultured on plates coated with inactivated PR8 virus as an in vitro antigenic stimulator (mean ± SD).