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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: New Phytol. 2011 Feb 23;190(4):906–915. doi: 10.1111/j.1469-8137.2011.03647.x

Fig. 1.

Fig. 1

miR169 is down-regulated by nitrogen (N) deficiency in roots (a) and shoots (b). Arabidopsis plants were grown hydroponically in a nutrient solution containing 0.5 mM Ca(NO3)2 for 5 wk and then transferred to N-free or N-replete medium for the indicated times before small RNA was isolated from leaves and roots. Five micrograms of small RNA from each sample was loaded per lane and hybridized with a 32P-labeled probe of ASRP1815. The blot reprobed with miR171 is shown as a loading control. The relative abundances in Arabidopsis plants grown in the N-free (closed bars) or N-replete (open bars) medium are shown in (c) (root) and (d) (shoot). *, P < 0.05 (t-test); significant difference from wild type (WT).