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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: Neuroscience. 2009 Aug 20;168(4):892–902. doi: 10.1016/j.neuroscience.2009.08.034

Fig. 3.

Fig. 3

Comparison of long and short-range diffusion of full-length AQPs and C-terminal deletion mutants. (A) Representative trajectories superimposed on cellular profiles (white regions) of COS-7 cells expressing AQP1 (left), AQP4-M1 (middle) or AQP4-M23 (right) determined from time-lapse SPT at 1 Hz over 6 min. (B) Combined MSD vs. time plots for AQP1 (grey), AQP4-M1 (black), AQP4-M23 (red), and deletion mutant M23Δ6 (blue). (C) Expansion of Fig. 3B with additional MSD plots for AQP4-M23 after treatment with latrunculin B (green), jasplakinolide (orange), nocodazole (purple) or paraformaldehyde (grey). (D) Average diffusion coefficients of AQP4-M1 (top group), AQP4-M23 (middle group), and AQP1 (bottom group) following indicated treatments or truncations. (E) P(range) for AQP1 (grey), AQP4-M1 (black), AQP4-M23 (red), and deletion mutants M23Δ6 (dark blue) and M1Δ6 (light blue). Data obtained from transiently transfected COS-7 cells. MSD plots (B and C) are results from time-lapse acquisition at 1 Hz over 6 min, while diffusion coefficients and ranges (D and E) were calculated following short-range measurements at 91 Hz over 6 s.