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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: Antiviral Res. 2011 Jun 14;91(2):195–208. doi: 10.1016/j.antiviral.2011.06.003

Figure 4. Influence of plasmid-derived expression levels on minigenome-derived reporter activity.

Figure 4

Filovirus minigenome assays were performed in presence or absence of RNA polymerase II inhibitors (α-amanitin and actinomycin D) at varying concentrations. The minigenome was based on EBOV and encoded renilla luciferase, whereas the control reporter for the level of plasmid-derived expression encoded firefly luciferase. Plotted are the renilla luciferase activity (minigenome-derived reporter activity) measurements as a function of firefly luciferase activity (plasmid derived gene expression), with both renilla and firefly luciferase activity in absence of inhibitors set to 100%. Shown are results from three independent experiments. Figure modified from (Hoenen et al., 2010b) with permission.