Figure 4. Influence of plasmid-derived expression levels on minigenome-derived reporter activity.
Filovirus minigenome assays were performed in presence or absence of RNA polymerase II inhibitors (α-amanitin and actinomycin D) at varying concentrations. The minigenome was based on EBOV and encoded renilla luciferase, whereas the control reporter for the level of plasmid-derived expression encoded firefly luciferase. Plotted are the renilla luciferase activity (minigenome-derived reporter activity) measurements as a function of firefly luciferase activity (plasmid derived gene expression), with both renilla and firefly luciferase activity in absence of inhibitors set to 100%. Shown are results from three independent experiments. Figure modified from (Hoenen et al., 2010b) with permission.