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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: Antiviral Res. 2011 Jun 14;91(2):195–208. doi: 10.1016/j.antiviral.2011.06.003

Table 1.

Available reverse genetics systems for negative-sense RNA viral hemorrhagic fever agents and closely related viruses.

Family Genus Virus Human disease Genome Minigenome systems trVLP systems Full-length clone system
Filoviridae Ebolavirus Ebola virus (EBOV) Ebola virus disease non-segmented negative sense (Mühlberger et al., 1999) (Watanabe et al., 2004)*, (Hoenen et al., 2006)** (Volchkov et al., 2001)
Reston virus (RESTV) none (Groseth et al., 2005)
Marburgvirus Marburg virus (MARV) Marburg virus disease (Mühlberger et al., 1998) (Wenigenrath et al., 2010)*, (Krähling et al., 2010)** (Enterlein et al., 2006)
Arenaviridae Arenavirus (Old World) Lassa virus (LASV) Lassa fever bi-segmented ambisense (Hass et al., 2004)
Arenavirus (New World) Junín virus (JUNV) Argentinian HF (Albarino et al., 2009) *** (Albarino et al., 2009)
Tacaribe virus (TCRV) none (Lopez et al., 2001) ***
Machupo virus (MACV) Bolivian HF (Kranzusch et al., 2010)
Bunyaviridae Orthobunyavirus Bunyamwera (BUNV) none tri-segmented ambisense or negative sense (Dunn et al., 1995) (Shi et al., 2006) (Bridgen and Elliott, 1996)
Phlebovirus Rift Valley fever virus (RVFV) Rift Valley fever (Ikegami et al., 2005) (Habjan et al., 2009a) (Ikegami et al., 2006)
Uukuniemi virus (UUKV) none (Flick and Pettersson, 2001) (Overby et al., 2006)
Hantavirus Hantaan virus (HTNV) HF with renal syndrome (Flick et al., 2003a)
Nairovirus Crimean-Congo hemorrhagic fever virus (CCHFV) Crimean Congo HF (Bergeron et al., 2010; Flick et al., 2003b)
*

System with pre-transfected target cells

**

System with naïve target cells

***

A chimeric approach using a combination of components from Tacaribe virus and Junin virus has been reported (Casabona et al., 2009)