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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Mech Behav Biomed Mater. 2012 Nov 10;18:67–80. doi: 10.1016/j.jmbbm.2012.10.014

Fig. 6.

Fig. 6

Effects of DMSO treatment on ECM microstructure for different freezing temperatures. (a) Scanning electron micrographs of frozen/thawed ECM microstructure for different concentrations of DMSO (scale bar = 5 μm) for TL = −20 °C. Significantly less alteration in ECM microstructure is observed when the DMSO concentration increases from 1 M to 2 M. (b) A similar threshold transition occurs for the freezing temperature of TL = −40 °C. The appearance of frozen/thawed ECM microstructure approaches that of unfrozen ECM when the DMSO concentration increases from 2 M to 3 M.