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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Inorg Biochem. 2012 Mar 3;110:8–17. doi: 10.1016/j.jinorgbio.2012.02.016

Fig. 1.

Fig. 1

cDDP binds and multimerizes MBD6. (A) ICP-MS analysis of cDDP binding when a two-fold molar excess was incubated with maltose binding protein (MBP), MBD6-SXXS and MBD6 for 5, 30 or 60 min. (B) Interaction of MBD6, MBD-SXXS and MBP with cDDP analyzed by gel electrophoresis following incubation for 1 h of the protein samples with increasing levels of cDDP as indicated. (C) Time course of interaction of MBD6 with a 25 molar excess cDDP analyzed by gel electrophoresis. (D) UV spectrometric analysis of the interaction of cDDP with MBD6. Absorption was measured at 280 nm at 60 s intervals for 2 h. (●), MBD6 in the absence of cDDP; (▼) MBP and (○) MBD6 in the presence of a 25 molar excess cDDP. Each data point in (A) is the average of 3 independent experiments.