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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: Biochemistry. 2009 Nov 3;48(43):10308–10320. doi: 10.1021/bi900980g

Figure 3.

Figure 3

(A) Binding isotherms for ScaR and duplex DNA containing operator sequences obtained from fluorescence anisotropy assays performed in Binding Buffer (25 mM HEPES, pH 7.5, 500 mM NaCl, 10% glycerol) with horizontal axis presented logarithmically. (▪) ScaR•Mn2+ binding to sc.51, (●) ScaR•Mn2+ binding to sc1.24, and (♦) Scar•Mn2+ binding to sc2.24. Curve fits assume 1:1 binding stoichiometry between ScaR and the DNA duplex, though ScaR binds in a 2:1 stoichiometry to sc.51. (B) Binding isotherm of ScaR with sc1.51 performed in Binding Buffer supplemented with 50 mM MgCl2. The data presented are the average of three trials. The curve is derived from a fit of the Hill Equation, with a Hill coefficient of 1.72 ± 0.14.