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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Proteome Res. 2013 Jan 28;12(3):1282–1288. doi: 10.1021/pr3009397

Figure 1.

Figure 1

SILAC-based quantitative proteomics. (A) Flowchart of reverse SILAC coupled with LC-MS/MS for the comparative analysis of protein expression in GM00637 cells following NNN treatment. In forward SILAC experiments, light Lys- and Arg-labeled cells were treated with 5 μM NNN, whereas the heavy Lys- and Arg-labeled cells were used as vehicle control. (B) A Venn diagram summarizing the number of proteins quantified from three independent SILAC (two forward and one reverse) experiments.