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. Author manuscript; available in PMC: 2014 Feb 25.
Published in final edited form as: Dev Cell. 2013 Feb 25;24(4):411–425. doi: 10.1016/j.devcel.2013.01.018

Figure 3. Distinct, lineage-restricted defects in hematopoiesis in rpl22 and rpl22l1 morphants.

Figure 3

(A–B) WISH analysis of thymocyte development with the indicated probes in rpl22 (A) and rpl22l1 morphants (B) at 5 dpf. Thymus, red dashed rectangles.

(C–E) Evaluation of thymus colonization and HSC emergence in rpl22l1 and rpl22 morphants. Tg(cd41:EGFP) embryos were injected with Rpl22l1 MO, Rpl22 MO, or MM control, following which seeding of the thymus and HSC emergence was assessed at 3.5dpf by tracking the presence of GFP+ cells in the thymus (red circles) or VDA (between the red and white dashed lines). Emerging HSC are indicated by arrows. The area between the dashed white and yellow lines is the pronephric duct. The number of CD41-EGFPlow HSCs was quantified in 6 representative embryos per group and presented as mean ± SD. *, p < 0.001.

Images depict phenotypes representative of at least 3 separate experiments, with numbers referring to the fraction of morphants with the depicted phenotypes. See also Figure S3.